Anti-Lumican/LUM Antibody Picoband®

SKU: A01034-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LUM

Specificity:

Background: Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.

Immunogen: E.coli-derived human NF45/ILF2 recombinant protein (Position: G21-K356). Human ILF2 shares 100% amino acid (aa) sequence identity with mouse?ILF2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chakravarti, S., Magnuson, T. Localization of mouse lumican (keratan sulfate proteoglycan) to distal chromosome 10. Mammalian Genome 6: 367-368, 1995. 2. Chakravarti, S., Stallings, R. L., SundarRaj, N., Cornuet, P. K., Hassell, J. R. Primary structure of human lumican (Keratan sulfate proteoglycan) and localization of the gene (LUM) to chromosome 12q21.3-q22. Genomics 27: 481-488, 1995. 3. Grover, J., Chen, X.-N., Korenberg, J. R., Roughley, P. J. The human lumican gene: organization, chromosomal location, and expression in articular cartilage. J. Biol. Chem. 270: 21942-21949, 1995.

Uniprot ID: P51884

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 4060

Protein Name: Lumican

Gene Full Name: lumican

Synonyms: KSPG lumican; LDC; LUM; lumican; SLRR2D