Anti-LDL Receptor/LDLR Antibody Picoband®

SKU: A00076-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Rat
ELISA, 0.1-0.5μg/ml, -

Gene Name: LDLR

Specificity: No cross reactivity with other proteins.

Background: In humans, the LDL receptor protein is encoded by the LDLR gene on chromosome 19. It is mapped to 19p13.2. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

Immunogen: E.coli-derived human LDL Receptor/LDLR recombinant protein (Position: Q35-D843).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Aalto-Setala, K., Helve, E., Kovanen, P. T., Kontula, K. Finnish type of low absorbance lipoprotein receptor gene mutation (FH-Helsinki) deletes exons encoding the carboxy-terminal part of the receptor and creates an internalization-defective phenotype. J. Clin. Invest. 84: 499-505, 1989. 2. Aalto-Setala, K., Koivisto, U.-M., Miettinen, T. A., Gylling, H., Kesaniemi, Y. A., Savolainen, M., Pyorala, K., Ebeling, T., Mononen, I., Turtola, H., Viikari, J., Kontula, K. Prevalence and geographical distribution of major LDL receptor gene rearrangements in Finland. J. Intern. Med. 231: 227-234, 1992. 3. Agnello, V., Abel, G., Elfahal, M., Knight, G. B., Zhang, Q.-X. Hepatitis C virus and other flaviviridae viruses enter cells via low absorbance lipoprotein receptor. Proc. Nat. Acad. Sci. 96: 12766-12771, 1999.

Uniprot ID: P01130

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 3949

Protein Name: Low-density lipoprotein receptor

Gene Full Name: low density lipoprotein receptor

Synonyms: FH; FHC; LDL receptor; LDLR