Anti-LCMT2 Antibody Picoband®

SKU: A14797-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LCMT2

Specificity:

Background: Leucine carboxyl methyltransferase 2 is an enzyme that in humans is encoded by the LCMT2 gene. The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW).

Immunogen: E.coli-derived human LCMT2 recombinant protein (Position: A29-N595).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 6/3/2013. 2. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. 3. Noma, A., Suzuki, T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. 50: 65-66, 2006.

Uniprot ID: O60294

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 76 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 9836

Protein Name: tRNA wybutosine-synthesizing protein 4

Gene Full Name: leucine carboxyl methyltransferase 2

Synonyms: LCMT2; KIAA0547; TYW4; tRNA wybutosine-synthesizing protein 4; tRNA yW-synthesizing protein 4; Leucine carboxyl methyltransferase 2; tRNA(Phe); 7-(3-amino-3-(methoxycarbonylpropylwyosine(37)-N)-methoxycarbonyltransferase; tRNA(Phe); 7-(3-amino-3-carboxypropylwyosine(37)-O)-methyltransferase