Anti-LCAT Antibody Picoband®

SKU: PA1967

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Rat,Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat

Gene Name: LCAT

Specificity: No cross reactivity with other proteins.

Background: LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed reverse cholesterol transport The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000).

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT, different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Azoulay, M., Henry, I., Tata, F., Weil, D., Grzeschik, K. H., Chaves, E., McIntyre, N., Williamson, R., Humphries, S. E., Junien, C. The structural gene for lecithin:cholesterol acyl transferase (LCAT) maps to 16q22. Ann. Hum. Genet. 51: 129-136, 1987. 2. Frohlich, J., McLeod, R., Hon, K. Lecithin:cholesterol acyl transferase (LCAT). Clin. Biochem. 15: 269-278, 1982. 3. Jonas, A. Lecithin cholesterol acyltransferase. Biochim. Biophys. Acta 1529: 245-256, 2000.

Uniprot ID: P04180

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight: 49578 MW

Gene ID: 3931

Protein Name: Phosphatidylcholine-sterol acyltransferase

Gene Full Name: lecithin-cholesterol acyltransferase

Synonyms: LCAT