Anti-JOSD2 Antibody Picoband®

SKU: A14517-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: JOSD2

Specificity:

Background: JOSD2 (Josephin-2) is a member of the Machado-Joseph Domain (MJD) deubiquitinating enzyme family. Other family members include: Ataxin-3, Ataxin-3L, and JOSD1. JOSD2 preferentially cleaves K63-linked ubiquitin chains, with much weaker activity toward K48-linked ubiquitin chains in vitro.

Immunogen: E.coli-derived human RTCA recombinant protein (Position: M1-D297). Human RTCA shares 92.3% amino acid (aa) sequence identity with mouse RTCA.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/21/2013. 2. Seki, T., Gong, L., Williams, A. J., Sakai, N., Todi, S. V., Paulson, H. L. JosD1, a membrane-targeted deubiquitinating enzyme, is activated by ubiquitination and regulates membrane dynamics, cell motility, and endocytosis. J. Biol. Chem. 288: 17145-17155, 2013.

Uniprot ID: Q8TAC2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 26 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 126119

Protein Name: Josephin-2

Gene Full Name: Josephin domain containing 2

Synonyms: JOSD2; SBBI54; Josephin-2; Josephin domain-containing protein 2