Anti-IPT/TRIT1 Antibody Picoband®

SKU: A08098

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TRIT1

Specificity:

Background: tRNA isopentenyltransferase, mitochondrial is an enzyme that in humans is encoded by the TRIT1 gene. This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional.

Immunogen: E.coli-derived human IPT/TRIT1 recombinant protein (Position: L16-K415).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Golovko, A., Hjalm, G., Sitbon, F., Nicander, B. Cloning of a human tRNA isopentenyl transferase. Gene 258: 85-93, 2000. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/19/2018. 3. Kernohan, K. D., Dyment, D. A., Pupavac, M., Cramer, Z., McBride, A., Bernard, G., Straub, I., Tetreault, M., Hartley, T., Huang, L., Sell, E., Majewski, J., and 14 others. Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene. Hum. Mutat. 38: 511-516, 2017.

Uniprot ID: Q9H3H1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 53 kDa

Calculated Molecular Weight: 17136 MW

Gene ID: 54802

Protein Name: tRNA dimethylallyltransferase

Gene Full Name: tRNA isopentenyltransferase 1

Synonyms: MOD5; tRNA dimethylallyltransferase; IPP transferase; IPPT; hGRO1; tRNA isopentenyltransferase 1; IPTase