Anti-ILKAP Antibody Picoband®

SKU: A08074-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ILKAP

Specificity:

Background: Integrin-linked kinase-associated serine/threonine phosphatase 2C is an enzyme that in humans is encoded by the ILKAP gene. The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Immunogen: E.coli-derived human PDK1 recombinant protein (Position: Y114-A436). Human PDK1 shares 94.7% and 95.7% amino acid (aa) sequence identity with mouse and rat PDK1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 6/10/2020. 2. Kumar, A. S., Naruszewicz, I., Wang, P., Leung-Hagesteijn, C., Hannigan, G. E. ILKAP regulates ILK signaling and inhibits anchorage-independent growth. Oncogene 23: 3454-3461, 2004. 3. Leung-Hagesteijn, C., Mahendra, A., Naruszewicz, I., Hannigan, G. E. Modulation of integrin signal transduction by ILKAP, a protein phosphatase 2C associating with the integrin-linked kinase, ILK1. EMBO J. 20: 2160-2170, 2001.

Uniprot ID: Q9H0C8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 43 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 80895

Protein Name: Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene Full Name: ILK associated serine/threonine phosphatase

Synonyms: ILKAP; Integrin-linked kinase-associated serine/threonine phosphatase 2C; ILKAP