Anti-IL5RA Antibody Picoband®

SKU: PA1619

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: IL5RA

Specificity: No cross reactivity with other proteins.

Background: IL5RA (INTERLEUKIN 5 RECEPTOR, ALPHA), also known as CD125, is a subunit of the Interleukin-5 receptor. IL5RA also denotes its human gene. The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The IL5RA gene is mapped to 3p26-p24 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines complemented by in situ hybridization. And its Cytogenetic location is 3p26.2. Within this 15-residue stretch of IL5RA, the C-terminal phenylalanine is critical. The binding of this protein to IL5 depends on the beta subunit which is activated by the ligand binding and is required for the biological activities of IL5.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cen, Osman; Gorska Magdalena M, Stafford Susan J, Sur Sanjiv, Alam Rafeul (Mar. 2003). Identification of UNC119 as a novel activator of SRC-type tyrosine kinases. 2. Geijsen, N., Uings, I. J., Pals, C., Armstrong, J., McKinnon, M., Raaijmakers, J. A. M., Lammers, J.-W. J., Koenderman, L., Coffer, P. J. Cytokine-specific transcriptional regulation through an IL-5R-alpha interacting protein. Science 293: 1136-1138, 2001. 3. Gough, N. M., Rakar, S. Localization of the IL-5 receptor gene to the distal half of murine chromosome 6 using recombinant inbred strains of mice. Genomics 12: 855-856, 1992.

Uniprot ID: Q01344

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 47685 MW

Gene ID: 3568

Protein Name: Interleukin-5 receptor subunit alpha

Gene Full Name: interleukin 5 receptor subunit alpha

Synonyms: CD125; CDw125; HSIL5R3; IL 5 receptor subunit alpha; IL 5R alpha; IL 5R subunit alpha; IL 5RA; IL5R; IL5RA; IL-5RA; interleukin 5 receptor; alpha