Anti-IkB Beta/NFKBIB Antibody Picoband®

SKU: A05766-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NFKBIB

Specificity:

Background: NF-kappa-B inhibitor beta is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human IkB Beta/NFKBIB recombinant protein (Position: T96-V356). Human NFKBIB shares 79.5% and 76.4% amino acid (aa) sequence identity with mouse and rat NFKBIB, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fenwick, C., Na, S.-Y., Voll, R. E., Zhong, H., Im, S.-Y., Lee, J. W., Ghosh, S. A subclass of Ras proteins that regulate the degradation of I-kappa-B. Science 287: 869-873, 2000. 2. Hoffmann, A., Levchenko, A., Scott, M. L., Baltimore, D. The I-kappa-B-NF-kappa-B signaling module: temporal control and selective gene activation. Science 298: 1241-1245, 2002. Note: Erratum: Science 318: 1550 only, 2007. 3. Lee, J. W., Choi, H.-S., Gyuris, J., Brent, R., Moore, D. D. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Molec. Endocr. 9: 243-254, 1995.

Uniprot ID: Q15653

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight: 38 kDa

Gene ID: 4793

Protein Name: NF-kappa-B inhibitor beta

Gene Full Name: NFKB inhibitor beta

Synonyms: I kappa B beta; IkappaBbeta; IkB B; IkB beta; IKBB; NF kappa B inhibitor beta; NF kappa BIB; NFKBIB; TR interacting protein 9; TRIP 9; TRIP9