Anti-Ikaros/IKZF1 Antibody Picoband® (monoclonal, 5F12H7)

SKU: M00531-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human

Gene Name: IKZF1

Specificity:

Background: DNA-binding protein Ikaros is a protein that in humans is encoded by the IKZF1 gene. This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros, different from the related mouse sequence by five amino acids.

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Georgopoulos K, Moore DD, Derfler B (December 1992). Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment. Science 258 (5083): 808–12. 2. Hahm K, Ernst P, Lo K, Kim GS, Turck C, Smale ST (November 1994). The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene. Mol Cell Biol 14 (11): 7111–23.

Uniprot ID: Q13422

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: IgG1

Phospho_site:

Clone Number: Clone: 5F12H7

Observed Molecular Weight: 55-65 kDa

Calculated Molecular Weight:

Gene ID: 10320

Protein Name: DNA-binding protein Ikaros

Gene Full Name: IKAROS family zinc finger 1

Synonyms: DNA binding protein Ikaros; hIk 1; Hs.54452; IK1; IKAROS; IKZF1; LYF1; PRO0758; ZNFN1A1