Anti-IFT52 Antibody Picoband®

SKU: A12872-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IFT52

Specificity:

Background: This gene encodes a conserved proline-rich protein that is a component of the intraflagellar transport-B (IFT-B) core complex. The encoded protein is essential for the integrity of the IFT-B core complex, and for biosynthesis and maintenance of cilia. Mutations in this gene are associated with ciliopathy that affects the skeleton.

Immunogen: E.coli-derived human LIS1/PAFAH1B1 recombinant protein (Position: I95-R410). Human PAFAH1B1 shares 100% amino acid (aa) sequence identity with both mouse and rat PAFAH1B1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, X., Wang, X., Jiang, C., Xu, M., Liu, Y., Qi, R., Qi, X., Sun, X., Xie, P., Liu, Q., Yan, B., Sheng, X., Zhao, C. IFT52 as a novel candidate for ciliopathies involving retinal degeneration. Invest. Ophthal. Vis. Sci. 59: 4581-4589, 2018. 2. Dupont, M. A., Humbert, C., Huber, C., Siour, Q., Guerrera, I. C., Jung, V., Christensen, A., Pouliet, A., Garfa-Traore, M., Nitschke, P., Injeyan, M., Millar, K., and 11 others. Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion. Hum. Molec. Genet. 28: 2720-2737, 2019. 3. Girisha, K. M., Shukla, A., Trujillano, D., Bhavani, G. S., Hebbar, M., Kadavigere, R., Rolfs, A. A homozygous nonsense variant in IFT52 is associated with a human skeletal ciliopathy. Clin. Genet. 90: 536-539, 2016.

Uniprot ID: Q9Y366

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 51098

Protein Name: Intraflagellar transport protein 52 homolog

Gene Full Name: intraflagellar transport 52

Synonyms: C20orf9; CGI 53; IFT52; NGD5; Protein NGD5 homolog