Anti-IFIT5 Antibody Picoband®

SKU: A07415-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IFIT5

Specificity:

Background: IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Immunogen: E.coli-derived human IFIT5 recombinant protein (Position: N23-S439).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abbas, Y. M., Pichlmair, A., Gorna, M. W., Superti-Furga, G., Nagar, B. Structural basis for viral 5-prime-PPP-RNA recognition by human IFIT proteins. Nature 494: 60-64, 2013. 2. Feng, F., Yuan, L., Wang, Y. E., Crowley, C., Lv, Z., Li, J., Liu, Y., Cheng, G., Zeng, S., Liang, H. Crystal structure and nucleotide selectivity of human IFIT5/ISG58. (Letter) Cell Res. 23: 1055-1058, 2013. 3. Gross, M. B. Personal Communication. Baltimore, Md. 12/18/2014.

Uniprot ID: Q13325

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 51200 MW

Gene ID: 24138

Protein Name: Interferon-induced protein with tetratricopeptide repeats 5

Gene Full Name: interferon induced protein with tetratricopeptide repeats 5

Synonyms: IFIT 5; IFIT5; RI58