Anti-IDI1 Antibody Picoband®

SKU: A07892-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IDI1

Specificity:

Background: IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity.

Immunogen: E.coli-derived human ATG7 recombinant protein (Position: D71-K645). Human ATG7 shares 92.9% amino acid (aa) sequence identity with both mouse and rat ATG7.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Breitling, R., Laubner, D., Clizbe, D., Adamski, J., Krisans, S. K. Isopentenyl-diphosphate isomerases in human and mouse: evolutionary analysis of a mammalian gene duplication. J. Molec. Evol. 57: 282-291, 2003. 2. Clizbe, D. B., Owens, M. L., Masuda, K. R., Shackelford, J. E., Krisans, S. K. IDI2, a second isopentenyl diphosphate isomerase in mammals. J. Biol. Chem. 282: 6668-6676, 2007. 3. Hahn, F. M., Xuan, J. W., Chambers, A. F., Poulter, C. D. Human isopentenyl diphosphate:dimethylallyl diphosphate isomerase: overproduction, purification, and characterization. Arch. Biochem. Biophys. 332: 30-34, 1996.

Uniprot ID: Q13907

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 26-28 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 3422

Protein Name: Isopentenyl-diphosphate Delta-isomerase 1

Gene Full Name: isopentenyl-diphosphate delta isomerase 1

Synonyms: IDI1; Isopentenyl-diphosphate Delta-isomerase 1; Isopentenyl pyrophosphate isomerase 1; IPP isomerase 1; IPPI1