Anti-Human CD3-FITC/CD19-PE Cocktail

SKU: GBS-FC0004

Size:100Tests

Storage:This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:

Target Synonym:

Research Areas:

Conjugation:FITC;PE

Host:

Species reactivity:Human

Application:FCM

Usage:For whole blood samples, add 5 μL Anti-Human CD3-FITC/CD19-PE Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×10⁶ dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-FITC/CD19-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.

Isotype:

Clonality:Monoclonal

Clone NO.:OKT-3;CB19

UNIProt ID:

Background:This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ) and PE Anti-Human CD19 Antibody [Clone: CB19] (Mouse IgG1, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD19 is a single-chain transmembrane glycoprotein expressed on B cells of all stages except plasma cells. It is a common marker for B cells. CD19 is also expressed in follicular dendritic cells. It forms complex with CD21 and CD84, which forms co-receptor with BCR. It takes part in B cell development, activantion and differentiation.

Concentration:5 μL/Test

Control catalog number:

Immunogen:

Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

Purification method:

Dilution:

GeneID: