Anti-HTATSF1 Antibody Picoband®

SKU: A07890-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HTATSF1

Specificity:

Background: HIV Tat-specific factor 1 is a protein that in humans is encoded by the HTATSF1 gene. The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human HTATSF1 recombinant protein (Position: H126-D333). Human HTATSF1 shares 96.2% amino acid (aa) sequence identity with mouse HTATSF1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fong, Y. W., Zhou, Q. Stimulatory effect of splicing factors on transcriptional elongation. Nature 414: 929-933, 2001. 2. Stumpf, A. M. Personal Communication. Baltimore, Md. 10/01/2020. 3. Zhang, Z., Will, C. L., Bertram, K., Dybkov, O., Hartmuth, K., Agafonov, D. E., Hofele, R., Urlaub, H., Kastner, B., Luhrmann, R., Stark, H. Molecular architecture of the human 17S U2 snRNP. Nature 583: 310-313, 2020.

Uniprot ID: O43719

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight:

Gene ID: 27336

Protein Name: 17S U2 SnRNP complex component HTATSF1

Gene Full Name: HIV-1 Tat specific factor 1

Synonyms: HIV 1 Tat specific factor 1; HIV Tat specific factor 1; HTATSF1; TAT SF1