Anti-Hsp40/DNAJB1 Antibody Picoband®

SKU: A03100-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: DNAJB1

Specificity:

Background: DnaJ homolog subfamily B member 1 is a protein that in humans is encoded by the DNAJB1 gene. This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human Hsp40/DNAJB1 recombinant protein (Position: M1-D313).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chan, H. Y. E., Warrick, J. M., Gray-Board, G. L., Paulson, H. L., Bonini, N. M. Mechanisms of chaperone suppression of polyglutamine disease: selectivity, synergy and modulation of protein solubility in Drosophila. Hum. Molec. Genet. 9: 2811-2820, 2000. 2. Hata, M., Ohtsuka, K. Characterization of HSE sequences in human Hsp40 gene: structural and promoter analysis. Biochim. Biophys. Acta 1397: 43-55, 1998. 3. Hata, M., Okumura, K., Seto, M., Ohtsuka, K. Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2. Genomics 38: 446-449, 1996.

Uniprot ID: P25685

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 64406 MW

Gene ID: 3337

Protein Name: DnaJ homolog subfamily B member 1

Gene Full Name: DnaJ heat shock protein family (Hsp40) member B1

Synonyms: DnaJ protein homolog 1; DNAJ1; DNAJB1; hDj 1; Hdj1; Heat shock 40 kDa protein 1; Heat shock protein 40; Hsp40; HSPF1; Human DnaJ protein 1; Sis1