Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6)

SKU: M08821-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: H1F0

Specificity:

Background: H1 histone family, member 0is a member of thehistonefamily of nuclearproteinswhich are a component ofchromatin. In humans, this protein is encoded by theH1F0gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Immunogen: E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Doenecke D, Tönjes R (February 1986). Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene. Journal of Molecular Biology. 187 (3): 461–4. 2. Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik KH, Doenecke D (June 1993). All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6. Genomics. 16 (3): 649–54.

Uniprot ID: P07305

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 5I3E6

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight:

Gene ID: 3005

Protein Name: Histone H1.0

Gene Full Name: H1.0 linker histone

Synonyms: H1 histone family; member 0; H10; H1F0; H1FV; histone; Histone H1; Histone H1(0); Histone H1.0