Anti-HIF1AN Antibody Picoband®

SKU: A02116-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: HIF1AN

Specificity:

Background: Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in negative regulation of Notch signaling pathway and positive regulation of myoblast differentiation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm.

Immunogen: E.coli-derived human HIF1AN recombinant protein (Position: D28-N349). Human HIF1AN shares 98.4% amino acid (aa) sequence identity with mouse HIF1AN.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hoff, S., Halbritter, J., Epting, D., Frank, V., Nguyen, T.-M. T., van Reeuwijk, J., Boehlke, C., Schell, C., Yasunaga, T., Helmstadter, M., Mergen, M., Filhol, E., and 29 others. ANKS6 is a central component of a nephronophthisis module linking NEK8 to INVS and NPHP3. Nature Genet. 45: 951-956, 2013. 2. Mahon, P. C., Hirota, K., Semenza, G. L. FIH-1: a novel protein that interacts with HIF-1-alpha and VHL to mediate repression of HIF-1 transcriptional activity. Genes Dev. 15: 2675-2686, 2001.

Uniprot ID: Q9NWT6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 40 kDa

Gene ID: 55662

Protein Name: Hypoxia-inducible factor 1-alpha inhibitor

Gene Full Name: hypoxia inducible factor 1 subunit alpha inhibitor

Synonyms: Factor inhibiting HIF 1; FIH 1; FIH1; HIF1AN