Anti-Hexokinase II/HK2 Antibody Picoband®

SKU: A01389

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: HK2

Specificity: No cross reactivity with other proteins.

Background: Hexokinase 2, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: HK2 hexokinase 2. 2. Printz RL, Osawa H, Ardehali H, Koch S, Granner DK (Feb 1997). Hexokinase II gene: structure, regulation and promoter organization. Biochemical Society Transactions. 25 (1): 107–12. 3. Peng Q, Zhou J, Zhou Q, Pan F, Zhong D, Liang H (2009). Silencing hexokinase II gene sensitizes human colon cancer cells to 5-fluorouracil. Hepato-Gastroenterology. 56 (90): 355–60.

Uniprot ID: P52789

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 102 kDa

Calculated Molecular Weight: 102 kDa

Gene ID: 3099

Protein Name: Hexokinase-2

Gene Full Name: hexokinase 2

Synonyms: Hexokinase 2; Hexokinase type II; HK II; HK2; HKII; HXK2; Muscle form hexokinase