Anti-HAP1 Antibody Picoband®

SKU: A01658-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: HAP1

Specificity:

Background: Huntingtin-associated protein 1 (HAP1) is a protein which in humans is encoded by the HAP1 gene. Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human HAP1 recombinant protein (Position: M1-R671).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bertaux, F., Sharp, A. H., Ross, C. A., Lehrach, H., Bates, G. P., Wanker, E. HAP1-huntingtin interactions do not contribute to the molecular pathology in Huntington's disease transgenic mice. FEBS Lett. 426: 229-232, 1998. 2. Chan, E. Y. W., Nasir, J., Gutekunst, C.-A., Coleman, S., Maclean, A., Maas, A., Metzler, M., Gertsenstein, M., Ross, C. A., Nagy, A., Hayden, M. R. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum. Molec. Genet. 11: 945-959, 2002. 3. Dragatsis, I., Zeitlin, S., Dietrich, P. Huntingtin-associated protein 1 (Hap1) mutant mice bypassing the early postnatal lethality are neuroanatomically normal and fertile but display growth retardation. Hum. Molec. Genet. 13: 3115-3125, 2004.

Uniprot ID: P54257

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 50514 MW

Gene ID: 9001

Protein Name: Huntingtin-associated protein 1

Gene Full Name: huntingtin associated protein 1

Synonyms: HAP 1; HAP1; HAP2; hHLP1; HIP5; HLP; HLP1; Neuroan 1