Anti-GYPB Antibody Picoband®

SKU: A05915-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: GYPB

Specificity:

Background: Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants.

Immunogen: E.coli-derived human GYPB recombinant protein (Position: Q43-A91).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Blumenfeld, O. O., Adamany, A. M. Structural (glycophorins) of the human erythrocyte membrane. Proc. Nat. Acad. Sci. 75: 2727-2731, 1978. 2. Cooling, L. Blood groups in infection and host susceptibility. Clin. Microbiol. Rev. 28: 801-870, 2015. 3. Divelbiss, J., Shiang, R., German, J., Moore, J., Murray, J. C., Patil, S. R. Refinement of the physical location of glycophorin A and beta fibrinogen using in situ hybridization and RFLP analysis. (Abstract) Cytogenet. Cell Genet. 51: 991, 1989.

Uniprot ID: P06028

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17 kDa

Calculated Molecular Weight: 10 kDa

Gene ID: 2994

Protein Name: Glycophorin-B

Gene Full Name: glycophorin B (MNS blood group)

Synonyms: GYPB; GPB; Glycophorin-B; PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; CD antigen CD235b