Anti-GTF2A2 Antibody Picoband®

SKU: A13029-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: GTF2A2

Specificity:

Background: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).

Immunogen: E.coli-derived human GTF2A2 recombinant protein (Position: M1-E109). Human GTF2A2shares 99.1% amino acid (aa) sequence identity with both mouse and rat GTF2A2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. DeJong, J., Bernstein, R., Roeder, R. G. Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription. Proc. Nat. Acad. Sci. 92: 3313-3317, 1995. 2. Upadhyaya, A. B., Khan, M., Mou, T.-C., Junker, M., Gray, D. M., DeJong, J. The germ cell-specific transcription factor ALF: structural properties and stabilization of the Tata-binding protein (TBP)-DNA complex. J. Biol. Chem. 277: 34208-34216, 2002. 3. Yudkovsky, N., Ranish, J. A., Hahn, S. A transcription reinitiation intermediate that is stabilized by activator. Nature 408: 225-229, 2000.

Uniprot ID: P52657

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17 kDa

Calculated Molecular Weight: 12 kDa

Gene ID: 2958

Protein Name: Transcription initiation factor IIA subunit 2

Gene Full Name: general transcription factor IIA subunit 2

Synonyms: TFIIA-12; TFIIA gamma; TFIIA 12; TFIIA; TF2A2