Anti-GSG2/HASPIN Antibody Picoband®

SKU: A31862-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: HASPIN

Specificity:

Background: Enables ATP binding activity and histone H3T3 kinase activity. Involved in several processes, including mitotic sister chromatid cohesion; mitotic spindle assembly checkpoint signaling; and protein localization to chromosome, centromeric region. Located in centrosome; nucleoplasm; and spindle.

Immunogen: E.coli-derived human GSG2/HASPIN recombinant protein (Position: R86-K798). Human GSG2/HASPIN shares 65% amino acid (aa) sequence identity with mouse GSG2/HASPIN.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dai, J., Sultan, S., Taylor, S. S., Higgins, J. M. G. The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes Dev. 19: 472-488, 2005. 2. Higgins, J. M. G. The haspin gene: location in an intron of the integrin alpha-E gene, associated transcription of an integrin alpha-E-derived RNA and expression in diploid as well as haploid cells. Gene 267: 55-69, 2001. 3. Kelly, A. E., Ghenoiu, C., Xue, J. Z., Zierhut, C., Kimura, H., Funabiki, H. Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B. Science 330: 235-239, 2010.

Uniprot ID: Q8TF76

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 88 kDa

Calculated Molecular Weight: 88 kDa

Gene ID: 83903

Protein Name: Serine/threonine-protein kinase haspin

Gene Full Name: histone H3 associated protein kinase

Synonyms: GSG2; H haspin; HASPIN