Anti-GPD1 Antibody Picoband®

SKU: A03262-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: GPD1

Specificity:

Background: This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GPD1. Human GPD1 shares 100% amino acid (aa) sequence identity with both mouse and rat GPD1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Basel-Vanagaite, L., Zevit, N., Zahav, A. H., Guo, L., Parathath, S., Pasmanik-Chor, M., McIntyre, A. D., Wang, J., Albin-Kaplanski, A., Hartman, C., Marom, D., Zeharia, A., Badir, A., Shoerman, O., Simon, A. J., Rechavi, G., Shohat, M., Hegele, R. A., Fisher, E. A., Shamir, R. Transient infantile hypertriglyceridemia, fatty liver, and hepatic fibrosis caused by mutated GPD1, encoding glycerol-3-phosphate dehydrogenase 1. Am. J. Hum. Genet. 90: 49-60, 2012. 2. Brown, L. J., Koza, R. A., Marshall, L., Kozak, L. P., MacDonald, M. J. Lethal hypoglycemic ketosis and glyceroluria in mice lacking both the mitochondrial and the cytosolic glycerol phosphate dehydrogenases. J. Biol. Chem. 277: 32899-32904, 2002. 3. Dionisi-Vici, C., Shteyer, E., Niceta, M., Rizzo, C., Pode-Shakked, B., Chillemi, G., Bruselles, A., Semeraro, M., Barel, O., Eyal, E., Kol, N., Haberman, Y., Lahad, A., Diomedi-Camassei, F., Marek-Yagel, D., Rechavi, G., Tartaglia, M., Anikster, Y. Expanding the molecular diversity and phenotypic spectrum of glycerol 3-phosphate dehydrogenase 1 deficiency. J. Inherit. Metab. Dis. 39: 689-695, 2016.

Uniprot ID: P21695

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 38 kDa

Gene ID: 2819

Protein Name: Glycerol-3-phosphate dehydrogenase [NAD

Gene Full Name: glycerol-3-phosphate dehydrogenase 1

Synonyms: GPDC; GPD C; GPD 1; Glycerol-3-phosphate dehydrogenase [NAD(+)]; cytoplasmic; EC:1.1.1.8