Anti-GNAI2 Antibody Picoband®

SKU: A02498-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GNAI2

Specificity:

Background: Guanine nucleotide-binding protein G(i), alpha-2 subunit is a protein that in humans is encoded by the GNAI2 gene. The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human GNAI2 recombinant protein (Position: R67-N312). Human GNAI2 shares 97.6% and 98% amino acid (aa) sequence identity with mouse and rat GNAI2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Blatt, C., Eversole-Cire, P., Cohn, V. H., Zollman, S., Fournier, R. E. K., Mohandas, L. T., Nesbitt, M., Lugo, T., Jones, D. T., Reed, R. R., Weiner, L. P., Sparkes, R. S., Simon, M. I. Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human. Proc. Nat. Acad. Sci. 85: 7642-7646, 1988. 2. Hermouet, S., Merendino, J. J., Jr., Gutkind, J. S., Spiegel, A. M. Activating and inactivating mutations of the alpha subunit of G(i2) protein have opposite effects on proliferation of NIH 3T3 cells. Proc. Nat. Acad. Sci. 88: 10455-10459, 1991. 3. Itoh, H., Toyama, R., Kozasa, T., Tsukamoto, T., Matsuoka, M., Kaziro, Y. Presence of three distinct molecular species of G(i) protein alpha subunit: structure of rat cDNAs and human genomic DNAs. J. Biol. Chem. 263: 6656-6664, 1988.

Uniprot ID: P04899

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 2771

Protein Name: Guanine nucleotide-binding protein G

Gene Full Name: G protein subunit alpha i2

Synonyms: GIP; GNAI2; GNAI2B; H_LUCA15.1; H_LUCA16.1