Anti-Glutathione Reductase/GSR Antibody Picoband®

SKU: A01479-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC-P,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry(Fixed), 1-3μg/1x10⁶ cells
ELISA, 0.1-0.5μg/ml

Gene Name: GSR

Specificity: No cross reactivity with other proteins.

Background: Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), is an enzyme that in humans is encoded by the GSR gene. This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Immunogen: E. coli-derived human Glutathione Reductase recombinant protein (Position: K256-R522).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Deponte M (May 2013). Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes. Biochim. Biophys. Acta. 1830 (5): 3217–66. 2. Meister A (November 1988). Glutathione metabolism and its selective modification. J. Biol. Chem. 263 (33): 17205–8.

Uniprot ID: P00390

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 38747 MW

Gene ID: 2936

Protein Name: Glutathione reductase, mitochondrial

Gene Full Name: glutathione-disulfide reductase

Synonyms: GLUR; glutathione reductase; GRase; GRD1; GSR