Anti-GAP43 Antibody (Monoclonal, GAP-7B10)

SKU: MA1042

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Gene Name: GAP43

Specificity: No cross reactivity with other proteins.

Background: GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

Immunogen: GAP-43 from neonatal rat forebrain membranes.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Kosik, K. S.; Orecchio, L. D.; Bruns, G. A. P.; Benowitz, L. I.; MacDonald, G. P.; Cox, D. R.; Neve, R. L. : Human GAP-43: its deduced amino acid sequence and chromosomal localization in mouse and human. Neuron 1: 127-132, 1988. 2. Strittmatter, S. M.; Fankhauser, C.; Huang, P. L.; Mashimo, H.; Fishman, M. C. : Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43. Cell 80: 445-452, 1995.

Uniprot ID: P07936

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: GAP-7B10

Observed Molecular Weight: 426 kDa

Calculated Molecular Weight: 23603 MW

Gene ID: Human(2596), Mouse(14432), Rat(29423)

Protein Name: Neuromodulin

Gene Full Name: growth associated protein 43

Synonyms: B-50; PP46; protein F1; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neural phosphoprotein B-50; neuron growth-associated protein 43