Anti-GANP/MCM3AP Antibody Picoband®

SKU: A05445-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MCM3AP

Specificity:

Background: 80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene. The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability.

Immunogen: E.coli-derived human GANP/MCM3AP recombinant protein (Position: R1275-H1715). Human MCM3AP shares 80.9% amino acid (aa) sequence identity with mouse MCM3AP.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abe, E., Kuwahara, K., Yoshida, M., Suzuki, M., Terasaki, H., Matsuo, Y., Takahashi, E., Sakaguchi, N. Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication. Gene 255: 219-227, 2000. 2. Karakaya, M., Mazaheri, N., Polat, I., Bharucha-Goebel, D., Donkervoort, S., Maroofian, R., Shariati, G., Hoelker, I., Monaghan, K., Winchester, S., Zori, R., Galehdari, H., Bonnemann, C. G., Yis, U., Wirth, B. Biallelic MCM3AP mutations cause Charcot-Marie-Tooth neuropathy with variable clinical presentation. (Letter) Brain 140: e65, 2017. Note: Electronic Article. 3. Kennerson, M. L., Corbett, A. C., Ellis, M., Perez-Siles, G., Nicholson, G. A. A novel MCM3AP mutation in a Lebanese family with recessive Charcot-Marie-Tooth neuropathy. (Letter) Brain 141: e66, 2018. Note: Electronic Article.

Uniprot ID: O60318

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 218 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 8888

Protein Name: Germinal-center associated nuclear protein

Gene Full Name: minichromosome maintenance complex component 3 associated protein

Synonyms: MCM3AP; GANP; KIAA0572; MAP80; Germinal-center associated nuclear protein; GANP; 80 kDa MCM3-associated protein; MCM3 acetylating protein; MCM3AP; MCM3 acetyltransferase