Anti-Gankyrin/PSMD10 Antibody Picoband®

SKU: A03169-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PSMD10

Specificity:

Background: This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

Immunogen: E.coli-derived human Gankyrin/PSMD10 recombinant protein (Position: M1-G226). Human Gankyrin/PSMD10 shares 94.2% and 94.7% amino acid (aa) sequence identity with mouse and rat Gankyrin/PSMD10, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dong, L., Yang, G., Pan, Y., Chen, Y., Tan, Y., Dai, R., Ren, Y., Fu, J., Wang, H. The oncoprotein p28(GANK) establishes a positive feedback loop in beta-catenin signaling. Cell Res. 21: 1248-1261, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 9/26/2012. 3. Hori, T., Kato, S., Saeki, M., DeMartino, G. N., Slaughter, C. A., Takeuchi, J., Toh-e, A., Tanaka, K. cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome. Gene 216: 113-122, 1998.

Uniprot ID: O75832

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight: 24 kDa

Gene ID: 5716

Protein Name: 26S proteasome non-ATPase regulatory subunit 10

Gene Full Name: proteasome 26S subunit, non-ATPase 10

Synonyms: PSMD10, p28(GANK), p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10