Anti-GADD34/Ppp1r15a Antibody Picoband®

SKU: A02394-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Ppp1r15a

Specificity:

Background: Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. Enables protein phosphatase binding activity and protein phosphatase regulator activity. Involved in several processes, including peptidyl-serine dephosphorylation; regulation of gene expression; and regulation of protein modification process. Acts upstream of or within endoplasmic reticulum unfolded protein response. Predicted to be located in several cellular components, including Golgi apparatus; cytosol; and mitochondrion. Predicted to be part of protein phosphatase type 1 complex. Predicted to be active in endoplasmic reticulum. Is expressed in several structures, including central nervous system; genitourinary system; gut; hemolymphoid system gland; and liver. Used to study thalassemia. Orthologous to human PPP1R15A (protein phosphatase 1 regulatory subunit 15A).

Immunogen: E.coli-derived mouse GADD34/Ppp1r15a recombinant protein (Position: R14-R572).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Blais, J. D., Filipenko, V., Bi, M., Harding, H. P., Ron, D., Koumenis, C., Wouters, B. G., Bell, J. C. Activating transcription factor 4 is translationally regulated by hypoxic stress. Molec. Cell. Biol. 24: 7469-7482, 2004. 2. Hasegawa, T., Yagi, A., Isobe, K. Interaction between GADD34 and kinesin superfamily, KIF3A. Biochem. Biophys. Res. Commun. 267: 593-596, 2000. 3. Hollander, M. C., Zhan, Q., Bae, I., Fornace, A. J., Jr. Mammalian GADD34, an apoptosis- and DNA damage-inducible gene. J. Biol. Chem. 272: 13731-13737, 1997

Uniprot ID: P17564

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight:

Gene ID: 17872

Protein Name: Protein phosphatase 1 regulatory subunit 15A

Gene Full Name: protein phosphatase 1, regulatory subunit 15A

Synonyms: GADD34; growth arrest and DNA damage-inducible protein 34; PPP1R15A