Anti-Eg5/KIF11 Antibody Picoband®

SKU: A01754-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: KIF11

Specificity:

Background: Kinesin-like protein KIF11 is a molecular motor protein that is essential in mitosis. In humans it is coded for by the gene KIF11. This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Immunogen: E.coli-derived human Eg5/KIF11 recombinant protein (Position: K17-Q716).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bartoli, K. M., Jakovljevic, J., Woolford, J. L., Jr., Saunders, W. S. Kinesin molecular motor Eg5 functions during polypeptide synthesis. Molec. Biol. Cell 22: 3420-3430, 2011. 2. Birtel, J., Gliem, M., Mangold, E., Tebbe, L., Spier, I., Muller, P. L., Holz, F. G., Neuhaus, C., Wolfrum, U., Bolz, H. J., Issa, P. C. Novel insights into the phenotypical spectrum of KIF11-associated retinopathy, including a new form of retinal ciliopathy. Invest. Ophthal. Vis. Sci. 58: 3950-3959, 2017. 3. Blangy, A., Lane, H. A., d'Herin, P., Harper, M., Kress, M., Nigg, E. A. Phosphorylation by p34(cdc2) regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 83: 1159-1169, 1995.

Uniprot ID: P52732

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 3832

Protein Name: Kinesin-like protein KIF11

Gene Full Name: kinesin family member 11

Synonyms: EG5; HKSP; KIF11; kinesin family member 11; Kinesin like protein 1; Kinesin like protein KIF11; KNSL1; TR interacting protein 5; TRIP 5; TRIP5