Anti-DHX29 Antibody Picoband®

SKU: A07866

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: DHX29

Specificity:

Background: DExH-box helicase 29 (DHX29) is a 155 kDa protein that in humans is encoded by the DHX29 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells.

Immunogen: E.coli-derived human DHX29 recombinant protein (Position: R56-K1343).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/1/2009. 2. Pisareva, V. P., Pisarev, A. V., Komar, A. A., Hellen, C. U. T., Pestova, T. V. Translation initiation on mammalian mRNAs with structured 5-prime UTRs requires DExH-box protein DHX29. Cell 135: 1237-1250, 2008.

Uniprot ID: Q7Z478

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 155 kDa

Calculated Molecular Weight: 49229 MW

Gene ID: 54505

Protein Name: ATP-dependent RNA helicase DHX29

Gene Full Name: DExH-box helicase 29

Synonyms: DDX29; DEAH box protein 29; DHX29; Nucleic acid helicase DDXx