Anti-DDX3/DDX3X Antibody Picoband®

SKU: A00751-6

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat

Gene Name: DDX3X

Specificity:

Background: ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene. The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3/DDX3X, identical to the related mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Lahn BT, Page DC (Nov 1997). Functional coherence of the human Y chromosome. Science. 278 (5338): 675–680. 2. Park SH, Lee SG, Kim Y, Song K (Oct 1998). Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3→p11.23. Cytogenet Cell Genet. 81 (3–4): 178–179. 3. Entrez Gene: DDX3X DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked.

Uniprot ID: O00571

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 73 kDa

Calculated Molecular Weight: 73243 MW

Gene ID: 1654

Protein Name: ATP-dependent RNA helicase DDX3X

Gene Full Name: DEAD-box helicase 3 X-linked

Synonyms: DBX; DDX14; DDX3; DDX3X; DEAD box; X isoform; Helicase like protein 2; HLP2