Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68)

SKU: MA1024

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, -
Western blot, 1-2μg/ml, Human

Gene Name: KRT7

Specificity: No cross reactivity with other proteins.

Background: KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

Immunogen: Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991. 2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002.

Uniprot ID: Q6IG12

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: LDS-68

Observed Molecular Weight: 37 kDa

Calculated Molecular Weight: 50709 MW

Gene ID: Human(3855), Mouse(110310), Rat(300242)

Protein Name: Keratin, type II cytoskeletal 7

Gene Full Name: keratin 7

Synonyms: SCL; cytokeratin 7; K2C7; K7; keratin; Keratin-7; 55K type II cytoskeletal; keratin, type II cytoskeletal 7; sarcolectin; KERATIN, SIMPLE EPITHELIAL; KERATIN, TYPE II, CYTOSKELETAL, 7; SARCOLECTIN; Type-II keratin Kb7