Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1)

SKU: M00672-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Gene Name: CYP2E1

Specificity:

Background: Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either ly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

Immunogen: E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kölble K (December 1993). Regional mapping of short tandem repeats on human chromosome 10: cytochrome P450 gene CYP2E, D10S196, D10S220, and D10S225. Genomics 18 (3): 702–4. 2. Hayashi S, Watanabe J, Kawajiri K (October 1991). Genetic polymorphisms in the 5'-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110 (4): 559–65. 3. Glew, Robert H. You Can Get There From Here: Acetone, Anionic Ketones and Even-Carbon Fatty Acids can Provide Substrates for Gluconeogenesis. Retrieved August 2013.

Uniprot ID: P05181

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 2C7G1

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight:

Gene ID: 1571

Protein Name: Cytochrome P450 2E1

Gene Full Name: cytochrome P450 family 2 subfamily E member 1

Synonyms: 4 nitrophenol 2 hydroxylase; CPE1; CYP2E; CYP2E1; CYP-2E1; CYP2E1-Specific; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 J; P450 J; P450C2E