Anti-Cyclin B1/CCNB1 Antibody Picoband®

SKU: A00745-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CCNB1

Specificity: No cross reactivity with other proteins.

Background: G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. It is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

Immunogen: E.coli-derived human Cyclin B1/CCNB1 recombinant protein (Position: M1-L383).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Matsuo, T., Yamaguchi, S., Mitsui, S., Emi, A., Shimoda, F., Okamura, H. Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255-259, 2003. 2. Milatovich, A., Francke, U. Human cyclin B1 gene (CCNB1) assigned to chromosome 5 (q13-qter). Somat. Cell Molec. Genet. 18: 303-307, 1992. 3. Moore, J. D., Kirk, J. A., Hunt, T. Unmasking the S-phase-promoting potential of cyclin B1. Science 300: 987-990, 2003.

Uniprot ID: P14635

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 51658 MW

Gene ID: 891

Protein Name: G2/mitotic-specific cyclin-B1

Gene Full Name: cyclin B1

Synonyms: CCNB; CCNB1; cyclin B1; G2/mitotic specific cyclin B1