Anti-CROP/LUC7L3 Antibody Picoband®

SKU: A08633-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LUC7L3

Specificity:

Background: LUC7 like 3 pre-mRNA splicing factor (LUC7L3), also known as Cisplatin resistance-associated overexpressed protein, or CROP, is a human gene. This gene encodes a protein with an N-terminal half that contains cysteine/histidine motifs and leucine zipper-like repeats, and the C-terminal half is rich in arginine and glutamate residues (RE domain) and arginine and serine residues (RS domain). This protein localizes with a speckled pattern in the nucleus, and could be involved in the formation of splicesome via the RE and RS domains. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen: E.coli-derived human CROP/LUC7L3 recombinant protein (Position: D30-H211).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 10/30/2017. 2. Nishii, Y., Morishima, M., Kakehi, Y., Umehara, K., Kioka, N., Terano, Y., Amachi, T., Ueda, K. CROP/Luc7A, a novel serine/arginine-rich nuclear protein, isolated from cisplatin-resistant cell line. FEBS Lett. 465: 153-156, 2000. 3. Umehara, H., Nishii, Y., Morishima, M., Kakehi, Y., Kioka, N., Amachi, T., Koizumi, J., Hagiwara, M., Ueda, K. Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A. Biochem. Biophys. Res. Commun. 301: 324-329, 2003.

Uniprot ID: O95232

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 51747

Protein Name: Luc7-like protein 3

Gene Full Name: LUC7 like 3 pre-mRNA splicing factor

Synonyms: LUC7L3; CREAP1; CROP; O48; Luc7-like protein 3; Cisplatin resistance-associated-overexpressed protein; Luc7A; Okadaic acid-inducible phosphoprotein OA48-18; cAMP regulatory element-associated protein 1; CRE-associated protein 1; CREAP-1