Anti-CLCN3 Antibody Picoband®

SKU: A05300-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CLCN3

Specificity:

Background: H+/Cl? exchange transporter 3 is a protein that in humans is encoded by the CLCN3 gene. This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen: E.coli-derived human CLCN3 recombinant protein (Position: G293-R752). Human CLCN3 shares 99.6% amino acid (aa) sequence identity with mouse CLCN3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Borsani, G., Rugarli, E. I., Taglialatela, M., Wong, C., Ballabio, A. Characterization of a human and murine gene (CLCN3) sharing similarities to voltage-gated chloride channels and to a yeast integral membrane protein. Genomics 27: 131-141, 1995. 2. Duncan, A. R., Polovitskaya, M. M., Gaitan-Penas, H., Bertelli, S., VanNoy, G. E., Grant, P. E., O'Donnell-Luria, A., Valivullah, Z., Lovgren, A. K., England, E. M., Agolini, E., Madden, J. A., and 28 others. Unique variants in CLCN3, encoding an endosomal anion/proton exchanger, underlie a spectrum of neurodevelopmental disorders. Am. J. Hum. Genet. 108: 1450-1465, 2021. 3. Mills, K. A., Mathews, K. D., Scherpbier-Heddema, T., Buetow, K. H., Baldini, A., Ballabio, A., Borsani, G. Genetic and physical mapping of a voltage-dependent chloride channel gene to human 4q32 and to mouse 8. Genomics 36: 374-376, 1996.

Uniprot ID: P51790

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 170 kDa

Calculated Molecular Weight:

Gene ID: 1182

Protein Name: H

Gene Full Name: chloride voltage-gated channel 3

Synonyms: CLCN3; H(+/Cl(- exchange transporter 3; Chloride channel protein 3; ClC-3; Chloride transporter ClC-3