Anti-Claudin 1/CLDN1 Antibody Picoband®

SKU: A01585-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CLDN1

Specificity:

Background: Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Immunogen: E.coli-derived human Claudin 1/CLDN1 recombinant protein (Position: F96-V211).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baala, L., Hadj-Rabia, S., Hamel-Teillac, D., Hadchouel, M., Prost, C., Leal, S. M., Jacquemin, E., Sefiani, A., de Prost, Y., Courtois, G., Munnich, A., Lyonnet, S., Vabres, P. Homozygosity mapping of a locus for a novel syndromic ichthyosis to chromosome 3q27-q28. J. Invest. Derm. 119: 70-76, 2002. 2. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003. 3. Dhawan, P., Singh, A. B., Deane, N. G., No, Y., Shiou, S.-R., Schmidt, C., Neff, J., Washington, M. K., Beauchamp, R. D. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J. Clin. Invest. 115: 1765-1776, 2005.

Uniprot ID: O95832

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 22 kDa

Calculated Molecular Weight: 34140 MW

Gene ID: 9076

Protein Name: Claudin-1

Gene Full Name: claudin 1

Synonyms: claudin 1; claudin1; CLD1; CLDN1; ILVASC; SEMP1