Anti-Chk1/CHEK1 Antibody Picoband®

SKU: A01060

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: CHEK1

Specificity: No cross reactivity with other proteins.

Background: CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.

Immunogen: E.coli-derived human Chk1 recombinant protein (Position: M1-Q210). Human Chk1 shares 96.7% and 97.6% amino acid (aa) sequence identity with mouse and rat Chk1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Flaggs, G., Plug, A. W., Dunks, K. M., Mundt, K. E., Ford, J. C., Quiggle, M. R. E., Taylor, E. M., Westphal, C. H., Ashley, T., Hoekstra, M. F., Carr, A. M. Atm-dependent interactions of a mammalian Chk1 homolog with meiotic chromosomes. Curr. Biol. 7: 977-986, 1997. 2. Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H., Elledge, S. J. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-1501, 1997. 3. Zhang, Y.-W., Otterness, D. M., Chiang, G. G., Xie, W., Liu, Y.-C., Mercurio, F., Abraham, R. T. Genotoxic stress targets human Chk1 for degradation by the ubiquitin-proteasome pathway. Molec. Cell 19: 607-618, 2005.

Uniprot ID: O14757

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 54434 MW

Gene ID: 1111

Protein Name: Serine/threonine-protein kinase Chk1

Gene Full Name: checkpoint kinase 1

Synonyms: CHEK1; CHK1