Anti-CEP250 Antibody Picoband®

SKU: A07150-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CEP250

Specificity:

Background: Centrosome-associated protein CEP250 is a protein that in humans is encoded by the CEP250 gene. This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human CEP250 recombinant protein (Position: Q563-K853).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bahe, S., Stierhof, Y.-D., Wilkinson, C. J., Leiss, F., Nigg, E. A. Rootletin forms centriole-associated filaments and functions in centrosome cohesion. J. Cell Biol. 171: 27-33, 2005. 2. de Castro-Miro, M., Tonda, R., Escudero-Ferruz, P., Andres, R., Mayor-Lorenzo, A., Castro, J., Ciccioli, M., Hidalgo, D. A., Rodriguez-Ezcurra, J. J., Farrando, J., Perez-Santonja, J. J., Cormand, B., Marfany, G., Gonzalez-Duarte, R. Novel candidate genes and a wide spectrum of structural and point mutations responsible for inherited retinal dystrophies revealed by exome sequencing. PLoS One 11: e0168966, 2016. Note: Electronic Article. 3. Fry, A. M., Mayor, T., Meraldi, P., Stierhof, Y.-D., Tanaka, K., Nigg, E. A. C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. J. Cell Biol. 141: 1563-1574, 1998.

Uniprot ID: Q9BV73

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight: 25503 MW

Gene ID: 11190

Protein Name: Centrosome-associated protein CEP250

Gene Full Name: centrosomal protein 250

Synonyms: 250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CNAP1, C-NAP1