Anti-Centromere protein R/ITGB3BP Antibody Picoband®

SKU: A09098-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ITGB3BP

Specificity:

Background: Centromere protein R is a protein that in humans is encoded by the ITGB3BP gene. This gene encodes a transcriptional coregulator that binds to and enhances the activity of members of the nuclear receptor families, thyroid hormone receptors and retinoid X receptors. This protein also acts as a corepressor of NF-kappaB-dependent signaling. This protein induces apoptosis in breast cancer cells through a caspase 2-mediated signaling pathway. This protein is also a component of the centromere-specific histone H3 variant nucleosome associated complex (CENP-NAC) and may be involved in mitotic progression by recruiting the histone H3 variant CENP-A to the centromere. Alternate splicing results in multiple transcript variants.

Immunogen: E.coli-derived human Centromere protein R/ITGB3BP recombinant protein (Position: M1-N177). Human ITGB3BP shares 94.4% and 59.8% amino acid (aa) sequence identity with mouse and rat ITGB3BP, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Li, D., Das, S., Yamada, T., Samuels, H. H. The NRIF3 family of transcriptional coregulators induces rapid and profound apoptosis in breast cancer cells. Molec. Cell. Biol. 24: 3838-3848, 2004. 2. Li, D., Desai-Yajnik, V., Lo, E., Schapira, M., Abagyan, R., Samuels, H. H. NRIF3 is a novel coactivator mediating functional specificity of nuclear hormone receptors. Molec. Cell. Biol. 19: 7191-7202, 1999. 3. Li, D., Wang, F., Samuels, H. H. Domain structure of the NRIF3 family of coregulators suggests potential dual roles in transcriptional regulation. Molec. Cell. Biol. 21: 8371-8384, 2001.

Uniprot ID: Q13352

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 20 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 23421

Protein Name: Centromere protein R

Gene Full Name: integrin subunit beta 3 binding protein

Synonyms: Beta 3 endonexin; CENP R; CENPR; Centromere protein R; HSU37139; ITGB3BP; NRIF3; TAP20