Anti-CDK1 Antibody (Monoclonal, A17)

SKU: MA1077

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Chicken,Human,Mouse

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 0.5-1μg/ml, Human, chicken, mouse

Gene Name: CDK1

Specificity: No cross reactivity with other proteins.

Background: P34 (cdc2), also known as cell division cycle (CDC2), or cyclin-dependent kinase 1 (CDK1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Immunogen: C-terminal two-thirds of Xenopus CDK1 expressed in E.coli.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Draetta, G.; Piwnica-Worms, H.; Morrison, D.; Druker, B.; Roberts, T.; Beach, D. : Human CDC2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336: 738-744, 1988. 2. Spurr, N. K.; Goodfellow, P. N.; Nurse, P.; Lee, M. : Assignment of the human homologue of the yeast cell cycle control gene CDC2 to chromosome 10. (Abstract) Cytogenet. Cell Genet. 46: 698, 1987. 3. Nazarenko, S. A.; Ostroverhova, N. V.; Spurr, N. K. : Regional assignment of the human cell cycle control gene CDC2 to chromosome 10q21 by in situ hybridization. Hum. Genet. 87: 621-622, 1991.

Uniprot ID: P39951

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: A17

Observed Molecular Weight: 18 kDa

Calculated Molecular Weight: 34135 MW

Gene ID: Human(983), Mouse(12534), Rat(54237)

Protein Name: Cyclin-dependent kinase 1

Gene Full Name: cyclin-dependent kinase 1

Synonyms: CDC2; CDC28A; CDKN1; P34CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase