Anti-CD35/CR1 Antibody Picoband®

SKU: A01022-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CR1

Specificity: No cross reactivity with other proteins.

Background: Complement receptor type 1 (CR1) also known as C3b/C4b receptor or CD35 (cluster of differentiation 35) is a protein that in humans is encoded by the CR1 gene. This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.

Immunogen: E.coli-derived human CD35/CR1 recombinant protein (Position: D109-Q957).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Jacobson AC, Weis JH (Sep 2008). Comparative functional evolution of human and mouse CR1 and CR2. J. Immunol. 181 (5): 2953–9. 2. Khera R, Das N (February 2009). Complement Receptor 1: Disease associations and therapeutic implications. Molecular Immunology. 46 (5): 761–772. 3. Moulds JM, Nickells MW, Moulds JJ, Brown MC, Atkinson JP (May 1991). The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. J. Exp. Med. 173 (5): 1159–63.

Uniprot ID: P17927

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight: 47579 MW

Gene ID: 1378

Protein Name: Complement receptor type 1

Gene Full Name: complement C3b/C4b receptor 1 (Knops blood group)

Synonyms: C3b/C4b receptor; C3BR; CD35; Complement receptor type 1; CR1; KN