Anti-CD3 epsilon/Cd3e Antibody Picoband®

SKU: A02765-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,IHC,WB,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5μg/ml, -, Rat

Gene Name: Cd3e

Specificity: No cross reactivity with other proteins.

Background: CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Immunogen: E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Clevers HC, Dunlap S, Wileman TE, Terhorst C (November 1988).Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter. Proc. Natl. Acad. Sci. U.S.A. 85(21): 8156–60. 2. Entrez Gene: CD3E CD3e molecule, epsilon (CD3-TCR complex). 3. Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C (1986). Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321 (6068): 431–4.

Uniprot ID: P22646

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 23 kDa

Calculated Molecular Weight: 12252 MW

Gene ID: 12501

Protein Name: T-cell surface glycoprotein CD3 epsilon chain

Gene Full Name: CD3 antigen, epsilon polypeptide

Synonyms: CD3; CD3 epsilon; CD3E; CD3-epsilon; T3E; TCRE