Anti-CaMKII alpha/CAMK2A Antibody Picoband®

SKU: A03241-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunofluorescence, 5μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Gene Name: CAMK2A

Specificity: No cross reactivity with other proteins.

Background: Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMKIIα), a.k.a. Ca2+/calmodulin-dependent protein kinase II alpha, is a protein kinase (i.e., an enzyme which phosphorylates proteins) that in humans is encoded by the CAMK2A gene. It is mapped to 5q32. The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca (2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen: E.coli-derived human CaMKII alpha/CAMK2A recombinant protein (Position: M1-H478).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Akita, T., Aoto, K., Kato, M., Shiina, M., Mutoh, H., Nakashima, M., Kuki, I., Okazaki, S., Magara, S., Shiihara, T., Yokochi, K., Aiba, K., Tohyama, J., Ohba, C., Miyatake, S., Miyake, N., Ogata, K., Fukuda, A., Matsumoto, N., Saitsu, H. De novo variants in CAMK2A and CAMK2B cause neurodevelopmental disorders. Ann. Clin. Transl. Neurol. 5: 280-296, 2018. 2. Bayer, K.-U., De Koninck, P., Leonard, A. S., Hell, J. W., Schulman, H. Interaction with the NMDA receptor locks CaMKII in an active conformation. Nature 411: 801-805, 2001. 3. Chen, C., Rainnie, D. G., Greene, R. W., Tonegawa, S. Abnormal fear response and aggressive behavior in mutant mice deficient for alpha-calcium-calmodulin kinase II. Science 266: 291-294, 1994.

Uniprot ID: Q9UQM7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight:

Gene ID: 815

Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha

Gene Full Name: calcium/calmodulin dependent protein kinase II alpha

Synonyms: CaM kinase II subunit alpha; CaMK II subunit alpha; CAMK2; CAMK2A; CAMK2B; CAMK2D; CAMK2G; CAMKA; CaMKII alpha; KIAA0968