Anti-CA12 Antibody Picoband®

SKU: A04063-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CA12

Specificity: No cross reactivity with other proteins.

Background: Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 15q22.2. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.

Immunogen: E.coli-derived human CA12 recombinant protein (Position: K45-A298).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hong, JH; Muhammad, E; Zheng, C; Hershkovitz, E; Alkrinawi, S; Loewenthal, N; Parvari, R; Muallem, S (15 December 2015). Essential role of carbonic anhydrase XII in secretory gland fluid and HCO3 (-) secretion revealed by disease causing human mutation. The Journal of Physiology. 593 (24): 5299–312. 2. Lee, M; Vecchio-Pagán, B; Sharma, N; Waheed, A; Li, X; Raraigh, KS; Robbins, S; Han, ST; Franca, AL; Pellicore, MJ; Evans, TA; Arcara, KM; Nguyen, H; Luan, S; Belchis, D; Hertecant, J; Zabner, J; Sly, WS; Cutting, GR (23 February 2016). Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease. Human Molecular Genetics. 25 (10): 1923–1933. 3. Almståhl, A; Wikström, M (May 2003). Electrolytes in stimulated whole saliva in individuals with hyposalivation of different origins. Archives of Oral Biology. 48 (5): 337–44. doi:10.1016/s0003-9969(02)00200-5. PMID 12711377. Manokaran, S; Zhang, X; Chen, W; Srivastava, DK (June 2010). Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine. Biochimica et Biophysica Acta. 1804 (6): 1376–84.

Uniprot ID: O43570

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight: 17184 MW

Gene ID: 771

Protein Name: Carbonic anhydrase 12

Gene Full Name: carbonic anhydrase 12

Synonyms: CA XII; CA12; Carbonate dehydratase XII; Carbonic anhydrase 12; carbonic anhydrase XII; CAXII; HsT18816; Tumor antigen HOM RCC 3.1.3