Anti-C19orf10/MYDGF Antibody Picoband®

SKU: A08602

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MYDGF

Specificity:

Background: Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis.

Immunogen: E.coli-derived human C19orf10/MYDGF recombinant protein (Position: V32-L173).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Tulin, E. E., Onoda, N., Nakata, Y., Maeda, M., Hasegawa, M., Nomura, H., Kitamura, T. SF20/IL-25, a novel bone marrow stroma-derived growth factor that binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation. J. Immun. 167: 6338-6347, 2001. Note: Retraction: J. Immun. 170: 1593 only, 2003. 2. Wang, P., Mariman, E., Keijer, J., Bouwman, F., Noben, J.-P., Robben, J., Renes, J. Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines. Cell. Molec. Life Sci. 61: 2405-2417, 2004. 3. Weiler, T., Du, Q., Krokhin, O., Ens, W., Standing, K., El-Gabalawy, H., Wilkins, J. A. The identification and characterization of a novel protein, c19orf10, in the synovium. Arthritis Res. Ther. 9: R30, 2007. Note: Electronic Article.

Uniprot ID: Q969H8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 15 kDa

Calculated Molecular Weight: 45470 MW

Gene ID: 56005

Protein Name: Myeloid-derived growth factor

Gene Full Name: myeloid derived growth factor

Synonyms: C19orf10; MYDGF; Myeloid-derived growth factor; SF20