Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4)

SKU: M00223-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Gene Name: SMARCA4

Specificity: No cross reactivity with other proteins.

Background: Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen: E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Entrez Gene: SMARCA4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4. 2. DiRenzo J, Shang Y, Phelan M, Sif S, Myers M, Kingston R, Brown M (October 2000). BRG-1 is recruited to estrogen-responsive promoters and cooperates with factors involved in histone acetylation. Molecular and Cellular Biology. 20 (20): 7541-9.

Uniprot ID: P51532

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 3F4

Observed Molecular Weight: 181 kDa

Calculated Molecular Weight:

Gene ID: 6597

Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

Gene Full Name: SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4

Synonyms: ATP dependent helicase SMARCA4; BAF190; BAF190A; BRG1; BRG1 associated factor 190A; hSNF2b; Protein brahma homolog 1; Protein BRG 1; SMARCA4; SMARCA4/BRG1; SNF2; SNF2 BETA; SNF2B; SNF2L4; SNF2LB; SWI2; Transcription activator BRG1