Anti-BCL11B Antibody Picoband®

SKU: A01485-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: BCL11B

Specificity:

Background: B-cell lymphoma/leukemia 11B is a protein that in humans is encoded by the BCL11B gene. This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen: E.coli-derived human BCL11B recombinant protein (Position: Q10-A628).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Arlotta, P., Molyneaux, B. J., Chen, J., Inoue, J., Kominami, R., Macklis, J. D. Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Neuron 45: 207-221, 2005. 2. Avram, D., Fields, A., Top, K. P. O., Nevrivy, D. J., Ishmael, J. E., Leid, M. Isolation of a novel family of C2H2 zinc finger proteins implicated in transcriptional repression mediated by chicken ovalbumin upstream promoter transcription factor (COUP-TF) orphan nuclear receptors. J. Biol. Chem. 275: 10315-10322, 2000. 3. Chen, B., Wang, S. S., Hattox, A. M., Rayburn, H., Nelson, S. B., McConnell, S. K. The Fezf2-Ctip2 genetic pathway regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. Proc. Nat. Acad. Sci. 105: 11382-11387, 2008.

Uniprot ID: Q9C0K0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 116 kDa

Calculated Molecular Weight: 75679 MW

Gene ID: 64919

Protein Name: B-cell lymphoma/leukemia 11B

Gene Full Name: BCL11 transcription factor B

Synonyms: B cell CLL/lymphoma 11B; B cell lymphoma/leukemia 11B; BCL11B; COUP TF interacting protein 2; CTIP 2; CTIP2; hRit1; hRIT1 alpha; RIT1